Cell type-specific role of Autophagy against intestinal inflammation

2014 Fiscal Year Final Research Report

• Project/Area Number: 25860568
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Gastroenterology
• Research Institution: Kobe Pharmaceutical University
• Principal Investigator: INOUE Jun 神戸薬科大学, 薬学部, 研究員 (50631561)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Keywords: オートファジー / 炎症性腸疾患 / 感染性腸炎

Outline of Final Research Achievements

Genome-wide association studies identified autophagy gene as a Crohn’s disease susceptibility gene. However it is unclear how autophagy regulates the inflammation in the intestine. The purpose of this study is to clarify the mechanism of autophagy to regulate the intestinal inflammation. We generated the organs specific autophagy deficient mouse, then the involvement of autophagy in intestinal inflammation was examined.
Secretion of IL-1β in the macrophages harvested from peritoneal cavity of the mice in which Atg7 had been conditionally deleted from the lysozyme cells was higher than that of control mice after infection with Citrobacter Rodentium, a pathogen used in the murine model of infectious enteritis. And more infected knockout mice exhibited greater clinical evidence of disease and higher expression levels of IL-1β mRNA in the large intestine. These results indicated that autophagy in the lysozyme cells have important role in the regulation of intestinal inflammation.

Free Research Field

医歯薬学