2014 Fiscal Year Final Research Report
Assessment of cardioprotective effect of PDE3A against chronic heart failure
| Project/Area Number |
25860613
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Cardiovascular medicine
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| Research Institution | Fukushima Medical University |
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Principal Investigator |
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| Project Period (FY) |
2013-04-01 – 2015-03-31
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| Keywords | Phosphodiesterase / Angiotensin II / Isoproterenol |
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| Outline of Final Research Achievements |
We used cardiospecific PDE3A overexpression (PDE3TG) mice to the study. Ischemia-reperfusion injury was subjected to PDE3TG or wild-type (WT) mice. PDE3TG mice showed smaller myocardial infarction area than WT mice. A number of apoptotic cariomyocytes were lower in PDE3TG mice. We also performed angiotensin II stimulation. PDE3TG mice displayed inhibited cardiac hypertrophy and fibrosis compared to WT mice. The expression levels of transforming growth factor β, which was increased in WT mice after angiotensin II stimulation, was inhibited in PDE3TG mice. Finally we performed Isoproterenol stimulation. PDE3TG mice showed inhibited cardiac hypertrophy compared to WT mice. 8-OHDG positive area, which indicates oxidative damage, was increased in WT mice heart after isoproterenol stimulation, but not in PDE3TG mice heart. Sirt1 protein expression levels were increased in PDE3TG heart. Thus, we conclude PDE3A has cardioprotective effects through various mechanisms.
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| Free Research Field |
循環器内科
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