2014 Fiscal Year Final Research Report
Analysis of microorganisms responsible for exacerbations of COPD by a semi-quantitative PCR method, HIRA-TAN
| Project/Area Number |
25860653
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Respiratory organ internal medicine
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| Research Institution | Saitama Medical University |
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Principal Investigator |
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| Project Period (FY) |
2013-04-01 – 2015-03-31
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| Keywords | COPD |
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| Outline of Final Research Achievements |
A prospective study was performed in 19 patients with COPD. Pathogenic bacteria were detected in induced sputa obtained from 7 COPD patients at stable conditions by culture (n=2) or a semi-quantitative PCR method, HIRA-TAN (Human cell controlled Identification of Respiratory Agent from “TAN”) (n=6). Viruses were not detected in any of the patients. The presence of bacteria was not related to symptoms or exacerbations during the observation period (mean 36 weeks). In a retrospective study, pathogenic bacteria in sputum were detected in 40 patients (34%) by culture and in 52 patients (45%) by the PCR method among COPD patients with exacerbations or pneumonia (n=115). It was noted that S. pneumonia was more frequently detected by HIRA-TAN (20%) than by culture (5%). Viruses were not investigated in the retrospective study. These observations suggest a potential benefit of the semi-quantitative PCR method, HIRA-TAN, to detect microorganisms potentially involved in exacerbations of COPD.
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| Free Research Field |
呼吸器内科
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