2014 Fiscal Year Final Research Report
Development of cell therapy using hiPS-derived nephron progenitors
| Project/Area Number |
25860674
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Kidney internal medicine
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| Research Institution | Kyoto University |
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Principal Investigator |
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| Project Period (FY) |
2013-04-01 – 2015-03-31
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| Keywords | 再生医学 / ネフロン前駆細胞 / 急性腎不全 / iPS細胞 |
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| Outline of Final Research Achievements |
In this study, we generated double reporter hiPSC lines, allowing us to monitor and isolate OSR1+SIX2+ nephron progenitors. We then established a multistep differentiation protocol from hiPSCs into OSR1+SIX2+ nephron progenitors with the induction efficiency nearly 40%. These human nephron progenitors differentiate into multiple renal epithelia including glomerular podocytes and tubular cells, and reconstitute three-dimensional renal structures in vitro and in vivo. Moreover, we found that renal subcapsular transplantation of hiPSC-derived OSR1+SIX2+ nephron progenitors ameliorated acute kidney injury in mice induced by ischemic reperfusion injury. Our results suggest that regenerative medicine strategies for kidney diseases could be developed using the hiPSC-derived renal cells.
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| Free Research Field |
再生医学、腎生理学
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