The therapeutic effect of mesenchymal stem cells cultured under serum-free media on kidney diseases: to apply them to clinical application

2014 Fiscal Year Final Research Report

• Project/Area Number: 25860681
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Kidney internal medicine
• Research Institution: Hiroshima University
• Principal Investigator: UENO TOSHINORI 広島大学, 大学病院, 医科診療医 (50646891)
• Research Collaborator: MASAKI Takao 広島大学病院, 教授 (30397913) ; DOI Shigehiro 広島大学病院, 助教 (80626127) ; NAKASHIMA Ayumu 広島大学病院, 特任助教 (40448262)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Keywords: 間葉系幹細胞 / 無血清培地 / 腎線維化

Outline of Final Research Achievements

Although anti-inflammatory and anti-fibrotic effects of mesenchymal stem cells (MSCs) transplanted into damaged tissues have been previously reported, almost all of them used serum-containing media and few studies have investigated using MSCs cultured under serum-free media. Here, we investigated a difference between the effect of MSCs cultured under serum-containing media and serum-free media. We administered both MSCs to a rat model of renal fibrosis. As a result of our investigation, renal inflammation and fibrosis were significantly suppressed in rats treated with serum-free MSCs compared with rats treated with serum-containing MSCs.

Free Research Field

腎臓