2014 Fiscal Year Final Research Report
Exploration of genes associated with efficacy of DNA methytransferase inhibitors for low risk MDS.
Project/Area Number |
25860781
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Multi-year Fund |
Research Field |
Hematology
|
Research Institution | The University of Tokyo |
Principal Investigator |
|
Co-Investigator(Renkei-kenkyūsha) |
YOSHIMI Akihide 東京大学, 医学部附属病院, 助教 (80609016)
|
Research Collaborator |
TSUKAMOTO Ayato
|
Project Period (FY) |
2013-04-01 – 2015-03-31
|
Keywords | 骨髄異形成症候群 / 抵抗性メカニズム / 全エクソームシークエンシング / shRNAライブラリ |
Outline of Final Research Achievements |
We conducted whole exome sequencing in patients with MDS and detected mutations specifically seen in azacitidine resistant phase, which were further narrowed down according to their functions and structures. Clone evolution during resistance acquisition was also analyzed with deep sequencing. Additionally, we screened responsible genes for azacitidine resistance using shRNA library system. We verified these candidate genes by demonstrating that suppression of the candidate genes conferred resistance to azacitidine in-vitro assays.
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Free Research Field |
造血器腫瘍
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