Establishment of the iPS cells derived from the paroxysmal nocturnal hemoglobinuria clone cells and its application to a study of bone marrow failure.

2014 Fiscal Year Final Research Report

• Project/Area Number: 25860785
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Hematology
• Research Institution: The University of Tokyo
• Principal Investigator: WATADANI Kumi 東京大学, 医学部附属病院, 助教 (70550432)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Keywords: 発作性夜間色素性尿症 / iPS細胞

Outline of Final Research Achievements

Establishment of induced pluripotent stem (iPS) cells derived from the affected cells of bone marrow failure including paroxysmal nocturnal hemoglobinuria is under investigation now. The object of this study is to establish the disease-specific iPS cells derived from PNH clone cells effectively and to promote elucidating the etiology of the disease by analyzing the hematopoietic cells differentiated from the iPS cells. We conducted trying induce iPS cells by reprogramming the PNH clone cells with CD55 negative and CD 59 negative populations of the bone marrow or the peripheral blood of classical PNH cases by use of Sendai virus vectors and non-viral episomal vectors.

Free Research Field

血液内科