Biological and pharmacological investigation on antigen-specific late asthmatic response

2014 Fiscal Year Final Research Report

• Project/Area Number: 25860823
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Collagenous pathology/Allergology
• Research Institution: Clinical Research Center for Allergy and Rheumatology, National Hospital Organization, Sagamihara National Hospital
• Principal Investigator: SEKIYA Kiyoshi 独立行政法人国立病院機構(相模原病院臨床研究センター), その他部局等, 研究員 (90385774)
• Research Collaborator: 森 晶夫 独立行政法人国立病院機構相模原病院臨床研究センター (80251247) ; 神山 智 独立行政法人国立病院機構相模原病院臨床研究センター (20626783)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Keywords: アレルギー / T細胞 / 気管支喘息 / 気流閉塞 / 平滑筋 / 遅発型反応

Outline of Final Research Achievements

In order to analyze T cell dependent airflow limitation, T cell clone transfer asthma model was established. Collagen gels embedded with primary cultured murine bronchial smooth muscle cells were established to detect contractile activity in vitro. Culture supernatant of the T cell clone that conferred late asthmatic response could induce gel contraction. The response was not inhibited by montelucast. However, montelucast inhibited late asthmatic response in vivo. T cell dependent airflow limitation seems to be induced through multiple pathways involved multiple mediators.

Free Research Field

呼吸器内科学、アレルギー学