2013 Fiscal Year Research-status Report
Patient-specific iPS cell-derived vessel smooth muscle cells of DD-EDS syndrome
| Project/Area Number |
25860853
|
|---|
| Research Category |
Grant-in-Aid for Young Scientists (B)
|
| Research Institution | Shinshu University |
|---|
Principal Investigator |
岳 鳳鳴 信州大学, 医学部, 助教 (20532865)
|
|---|
| Project Period (FY) |
2013-04-01 – 2016-03-31
|
| Keywords | patient- iPS cells / VSMCs / cardiomyocyte / disease model |
|---|
| Research Abstract |
D4ST1-deficient Ehlers-Danlos syndrome (DD-EDS, Kosho type) is a rare heterogeneous connective tissue disorder. Patients suffer from serious subcutaneous hematoma which is possible caused by insufficient contraction of the arterial smooth muscle. In this project, we investigate mechanism of hematoma using with using patient-specific iPS cell-derived vessel smooth muscle cells (VSMCs).
In research plan for 2013, I made a plan to induce vessel smooth muscle cells from patient-specific iPS cells. According to this plan, we induced the differentiation of DD-EDS-specific iPS cells and normal iPS cells into origin-specific VSMCs, and identified iPS cell-derived VSMCs by immunocytochemistry method.
In addition, in order to make circulation-system model, I also successfully induced the differentiation of patient-specific iPS-derived cardiomyocyte.
|
| Current Status of Research Progress |
Current Status of Research Progress
2: Research has progressed on the whole more than it was originally planned.
Reason
According to the application form innitially planned, I should induced the differentiation of vessel smooth muscle cells and compared compared differentiation efficiency in patient-iPS and normal iPS cells.
During 2013, I finally induced the VSMCs from not only normal iPS cells but also patient specific-iPS cells successfully, although I failed it for several times using with two kinds of methods which had beem published by other reseacheres.
In addition, in order to made disease model for circulation system research, I induced the differentiation of cardiomyocyte from patient-specific iPS cells.
Therefore, so far, I thoughtthat the whole project is processing smoothly, although I run across some difficulty when inducing the differentiation of smooth muscle cells and cardiomyocyte from iPS cells at the beginning stage.
|
| Strategy for Future Research Activity |
Since I have induced the differentiaiton of VSMCs from patient-specific iPS cells successfully, in the following 2014 year, I plan to compare the difference of iPS-derived VSMCs from patient-iPS and normal iPS cells, including the differentiation deficiency and function of smooth muscle cells, especially, contractile protein, contractile in response to pharmacological agonists such as carbachol or KCl, and vessel formation in matrigel plugs.
|
Research Products
(4 results)
-
-
-
[Presentation] Neural Model from iPS cells from DD-EDS patient.2013
Author(s)
Fengming Yue, Daihachiro Tomotsune, Hinako Ichikawa, Susumu Yoshie, Akimi Mogi, Yuto Takahashi, Shoko Masuda, Sakiko Shirasawa, Tadayuki Yokoyama, Mika Nagai, Katsunori Sasaki.
Organizer
The 11th ISSCR Annual Meeting
Place of Presentation
Boston, USA
Year and Date
20130613-20130616
-
