The analysis of TLR10 signaling pathway for elucidating the pathogenesis of allergic diseases.

2015 Fiscal Year Final Research Report

• Project/Area Number: 25860854
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Pediatrics
• Research Institution: Gifu University
• Principal Investigator: Kubota Kazuo 岐阜大学, 医学部附属病院, 助教 (10526940)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: 自然免疫 / Toll様受容体

Outline of Final Research Achievements

The aim of this study was to elucidate the pathophysiology of allergic diseases associated with Toll like receptor (TLR) signaling through basic research by proteomics analysis of human TLR10. We have attempted to establish an in vitro experimental system to mimic the interaction between intracellular Toll/IL-1 receptor (TIR) domains of TLR family including TLR10. The production and the analysis of the chimeric TIR proteins derived from different TLRs fused with an additional domain to N-terminus for forced dimerization was performed to investigate whether these fusion proteins possess bioactivity. First, the optimization experiment was performed using TLR1 and TLR2. In the similar condition, TLR2 combined with TLR6 could activate NF-κB signaling. On the other hand, the NF-κB activation induced by TLR2 combined with TLR10 was not observed. These results suggest that TLR10 may play as a potent negative regulator in TLR2 signaling pathway.

Free Research Field

自然免疫