The study on the mechanisms for efficacy of granulocyte adsorption apheresis in neutrophilic dermatosis

2014 Fiscal Year Final Research Report

• Project/Area Number: 25860943
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Dermatology
• Research Institution: Gifu University
• Principal Investigator: FUJISAWA TOMOMI 岐阜大学, 医学(系)研究科(研究院), 助教 (20585583)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Keywords: 顆粒球単球吸着療法 / 膿疱性乾癬 / 抗中球性皮膚症 / 樹状細胞 / 制御性T細胞 / ケモカイン / サイトカイン

Outline of Final Research Achievements

The mechanisms for efficacy of granulocyte adsorption apheresis(GMA) in neutrophilic dermatosis including pyoderma gangrenosum and generalized pustular psoriasis (GPP) were studied. We investigated the effects of GMA on the ratio of CD14+CD16+ proinflammatory monocytes/CD14+ monocytes and cytokine/chemokine production by these leukocytes before and after GMA sessions. Consequently, CD14+CD16+ monocytes were significantly increased, and the serum levels of CXCL8, CCL3 and CCL4 were elevated in patients with active GPP compared to healthy control and patient with quiescent GPP. GMA markedly reduced the CD14+CD16+/CD14+ ratio together with improvement of patients' clinical symptoms. Based on these results, we believe that in addition to neutrophils, elevated CD14+CD16+ proinflammatory monocytes are part of the immune pathology in GPP. Accordingly, selective depletion of CD14+CD16+ monocytes by GMA should be a therapeutic target in this condition.

Free Research Field

好中球性皮膚症