Evaluation of percutaneous immune responses and role of natural moisturizing factor in filaggrin monomer transgenic mice

2014 Fiscal Year Final Research Report

• Project/Area Number: 25860944
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Dermatology
• Research Institution: Hamamatsu University School of Medicine
• Principal Investigator: IKEYA Shigeki 浜松医科大学, 医学部附属病院, 助教 (40436936)
• Co-Investigator(Kenkyū-buntansha): TOKURA Yoshiki 浜松医科大学, 医学部, 教授 (00172156) ; SAKABE Jun-ichi 浜松医科大学, 医学部, 特任助教 (30631494)
• Research Collaborator: 高橋 慶人 株式会社カネボウ化粧品, 主任研究員 ; 行 卓男 株式会社カネボウ化粧品, 研究員
• Project Period (FY): 2013-04-01 – 2015-03-31
• Keywords: フィラグリン / 天然保湿因子 / アトピー性皮膚炎 / 皮膚バリア / 接触性皮膚炎

Outline of Final Research Achievements

We generated a FLG monomers transgenic mouse under the control of involucrin promoter. The FLG-TG mouse could have more filaggrin monomers and its derivatives, NMF. We speculate that our mice are more protective against outer stimuli and are prone to develop both irritant and allergic dermatitis. This type of mice revealed decreased transepidermal water loss and prevent the out-to-inside transpenetration of a low molecular weight chromatophore, Lucifer yellow. These findings clearly indicate the importance of filaggrin monomers in skin barrier. On the other hand, SC hydration was not up-regulated in FLG-TG mice. The amount of NMF, which could be expected to correlate with SC hydration, is dependent on proteases including bleomycin hydrolase. Thus, we thought that the amount of NMF in our transgenic mice need careful assessment. In the future plan, we would like to analyze FLG-TG mice mainly focusing on the immunological reaction.

Free Research Field

皮膚免疫、皮膚バリア機能、アトピー性皮膚炎、紫外線誘発皮膚癌の発症機序