2015 Fiscal Year Final Research Report
Establishment of domain specific Enzyme-Linked ImmunoSorbent Assay for pemphigus using recombinant proteins expressed by mammalian cells.
| Project/Area Number |
25860973
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Dermatology
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| Research Institution | Kurume University |
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Principal Investigator |
OHYAMA Bungo 久留米大学, 医学部, 講師 (90461441)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Keywords | 自己免疫性水疱症 / エピトープ解析 / ドメイン特異的ELISA法 / 哺乳類細胞発現系組み換え蛋白質 / 昆虫細胞発現系組み換え蛋白質 |
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| Outline of Final Research Achievements |
Pemphigus is autoimmune blistering disease caused by autoantibodies. Autoantibodies react with extracellular domains of desmoglein (Dsg) 1, Dsg3, desmocollin 1, and Dsc3 as antigens, and then cause blisters by abolished the adhesion between Dsg and Dsc proteins.
We attempted to establish domain specific Enzyme-Linked ImmunoSorbent Assay for pemphigus using recombinant proteins expressed by mammalian cells, because recombinant proteins of mammalian expression system keeps higher-order structures of protein than baculovirus insect expression system. We released some interesting data about epitopes which autoantibodies reacted against Dsg EC domains in three reports, and have established of IgA ELISAs of mammalian recombinant proteins of human Dsc 1-3.
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| Free Research Field |
皮膚科学
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