Possible molecular mechanisms of normal cells and invasive breast cancer cells after radiation treatment

2015 Fiscal Year Final Research Report

• Project/Area Number: 25861050
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Radiation science
• Research Institution: Hokkaido University
• Principal Investigator: NAM JINMIN 北海道大学, 国際連携研究教育局, 助教 (60414132)
• Research Collaborator: ONODERA YASUHITO 北海道大学, 大学院医学研究科, 講師 (90435561)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: 放射線 / 乳癌 / ３次元培養

Outline of Final Research Achievements

In this study, we investigated molecular mechanisms to improve the radiation therapy by targeting specific molecules which are involved in not only preservation of the functions in normal cell structure but also suppressing tumor growth, recurrence and metastasis. To find candidate genes, we performed microarray analysis in a mammary epithelial cell line which has basal polarity, or invasive breast cancer cell lines in three-dimensional laminin rich extracellular matrix (3D lrECM). We found that β1-integrin and its downstream molecules are involved in the disruption of basal polarity structure and invasive transition on non-malignant mammary epithelial cells in 3D lrECM. In addition, we also found that up-regulated integrin expression on the cell surface may have important roles in the invasive activity after radiation treatment in breast cancer cells.

Free Research Field

医歯薬学