2015 Fiscal Year Final Research Report
Increase in cell motility by carbon ion irradiation via the Rho signaling pathway and its inhibition by the ROCK inhibitor Y-27632 in lung adenocarcinoma
| Project/Area Number |
25861071
|
|---|
| Research Category |
Grant-in-Aid for Young Scientists (B)
|
| Allocation Type | Multi-year Fund |
|---|
| Research Field |
Radiation science
|
|---|
| Research Institution | Gunma University |
|---|
Principal Investigator |
|
|---|
| Project Period (FY) |
2013-04-01 – 2016-03-31
|
| Keywords | ヒト肺腺癌細胞 / 炭素線照射 / 遊走能 / Rho / ROCK |
|---|
| Outline of Final Research Achievements |
This study aimed to investigate the effect of carbon ion (C-ion) irradiation on cell motility through the Rho signaling pathway in the human lung adenocarcinoma cell line A549. At 48 h after irradiation, the wound-healing assay demonstrated that migration was significantly greater in cells irradiated with C-ion than in unirradiated cells. Similarly, F-actin staining showed that the formation of protrusions was significantly increased in cells irradiated with C-ion compared with unirradiated cells. Western-blot analysis showed that C-ion irradiation increased P-MLC2 expression compared with in unirradiated controls. Exposure to a non-toxic concentration of Y-27632, a specific inhibitor of ROCK, reduced the expression of P-MLC2 after C-ion irradiation, resulting in a significant reduction in migration. These data suggest that C-ion irradiation increases cell motility in A549 cells via the Rho signaling pathway and that ROCK inhibition reduces that effect.
|
| Free Research Field |
放射線腫瘍学
|
