Research of relationship between carbon ion beam resistance and heterochromatin formation in cancer cells

2015 Fiscal Year Final Research Report

• Project/Area Number: 25861141
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Radiation science
• Research Institution: National Institute of Radiological Sciences
• Principal Investigator: Sato Katsutoshi 国立研究開発法人放射線医学総合研究所, 重粒子医科学センター, 研究員 (20589650)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: 粒子線治療 / 炭素イオン線抵抗性

Outline of Final Research Achievements

Radioresistant cancer cell line X60 and C30 was originally established by means of repeated exposure the mouse squamous cell carcinoma cell line NR-S1 to X-ray and carbon ion beam (C-ion) irradiation, respectively. The results showed that the X60 cells acquired the resistance to both X-ray and C-ion irradiation. On the other hand, the X-ray and C-ion sensitivity of C30 cells was approximately same as that of NR-S1 cells. Moreover, it is shown that the X-ray and C-ion resistance was strongly correlated with the heterchromatin domain number, and the Rad51 focus formation was significantly promoted in X-ray and C-ion resistant X60 cells compared with that in NR-S1 and C30 cells. Our results suggested that homologous recombination repair in heterochromatin region is associated with the X-ray and C-ion resistance in cancer cells.

Free Research Field

医歯薬