The effect of immunosuppressive anergy cells on transplant arteriosclerosis in humanized mouse model

2014 Fiscal Year Final Research Report

• Project/Area Number: 25861146
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: General surgery
• Research Institution: Hokkaido University
• Principal Investigator: GOTO Ryoichi 北海道大学, 大学病院, 医員 (10645287)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Keywords: 抗原特異的 / 免疫抑制細胞 / ヒト化マウス / NSGマウス / 血管移植モデル

Outline of Final Research Achievements

We evaluated the effect of donor antigen specific immunosuppressive anergy cells on chronic rejection such as transplant arteriosclerosis. First, we confirmed that the immunosuppressive anergy cells were successfully generated from lymphocytes of healthy volunteer. Next, the severe immunodeficient NSG mice were reconstituted human PBMC and investigated the proliferation of human PBMC in vivo by CFSE labeling technique. Also when another irradiated human lymphocytes were injected into reconstituted mice as a donor antigen stimulation, the immune responses were observed by Ki-67 staining after stimulation. Further, to establish a human vessel transplant model, murine aortic transplant model has been established. In addition we evaluated the relationship between anti-donor antigen antibody and chronic rejection in liver transplant clinical setting.

Free Research Field

移植免疫