Methodology of the protein expression in the cardiac graft outside the body

2015 Fiscal Year Final Research Report

• Project/Area Number: 25861147
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: General surgery
• Research Institution: Hokkaido University
• Principal Investigator: Wakayama Kenji 北海道大学, 大学病院, 医員 (50646544)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: 移植・再生医療 / 虚血再灌流 / 臓器保存 / 酸化ストレス

Outline of Final Research Achievements

To enlarge donor pool for the heart transplantation, reliable method to repair the "less-than-ideal grafts". In the present study, we evaluated the merit and demerit of the oxidative phosphorylation under hypothermia. The results are summarized as follows. 1) Oxidative phosphorylation is progressed under hypothermic conditions. 2) The energy source of the ATP production under hypothermic oxygenated conditions is endogenous amino acids derived from the autophagic pathway. 3) This oxidative phosphorylation under hypothermia worsen the cellular death due to the oxidative stress. 4) Appropriate antioxidant therapy may reduced this cellular death. 5) Some proteins, probably chaperon, may actively regulate oxidative phosphorylation and autophagy under hypothermia.

Free Research Field

移植・再生医療