Examination of the effectiveness using the humanized mouse of the antiHER2 antibody epitope peptide vaccine.

2014 Fiscal Year Final Research Report

• Project/Area Number: 25861165
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: General surgery
• Research Institution: Tokai University
• Principal Investigator: TSUDA Banri 東海大学, 医学部, 助教 (30514533)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Keywords: 乳癌 / ペプチドワクチン / CH401MAP

Outline of Final Research Achievements

① After in vitro stimulating breast cancer patient PBMC (31) in CH401MAP, and comparing the production of the specific antibody with the physically unimpaired person, production was significantly high by non-stimulation in the patient group, but the change by the stimulation was not observed. ② After transfusing patient PBMC into the mouse, and immunising it in CH401MAP, I was able to build human immune system in acknowledgment of production of human IL-2. One GVHD did not occur. The usefulness as the preclinical test mouse on observing immune system was shown. ③ I transplanted a cancer tissue piece of two breast cancer patients, but was not able to engraft into the mouse. I ported human breast cancer cell strain SKBR3, but the survival was impossible. Furthermore, the need to make condition examination was shown.

Free Research Field

医歯薬学