New biomarker search and drug sensitivity prediction by the microRNA analysis of the GIST cultured cell

2014 Fiscal Year Final Research Report

• Project/Area Number: 25861177
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Digestive surgery
• Research Institution: University of Toyama
• Principal Investigator: MATSUI Koshi 富山大学, 大学院医学薬学研究部(医学), 助教 (20422636)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Keywords: GIST / マイクロRNA

Outline of Final Research Achievements

1)We were able to analyze the microRNA from a GIST operation cases, and two microRNA of miR146b-5p and miR335 aggravated in before and after Imatinib dosage. 2)The establishment of the GIST cultured cell was enabled. 3)GIST cell strain was transfused into the nude mouse and we collected a blood sample from the mouse subcutaneous transplant model and confirm an expression of microRNA change. 4)We collected the blood sample of the GIST patient and detected the microRNA. However, agreement was not seen in expression of microRNA detected by a result and a blood sample of the microRNA obtained from the excision specimen.

Free Research Field

消化器外科