2014 Fiscal Year Final Research Report
Novel assessment of hepatic iron distribution by synchrotron radiation X-ray fluorescence microscopy
| Project/Area Number |
25861189
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Digestive surgery
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| Research Institution | Kobe University |
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Principal Investigator |
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| Research Collaborator |
FUKUMOTO Takumi 神戸大学, 医学研究科, 准教授 (70379402)
KURAMITU Kaori 神戸大学, 医学部附属病院, 特定助教 (80596784)
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| Project Period (FY) |
2013-04-01 – 2015-03-31
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| Keywords | 放射線 |
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| Outline of Final Research Achievements |
Excess iron deposition in the liver is known to be hepatotoxic and may exacerbate liver injury. However, little is known about iron distribution in the lobule because of the lack of a highly sensitive detection method. The aim of this study is to determine iron distribution in the lobule of human liver by means of synchrotron radiation X-ray fluorescence (SRXRF) microscopy. Mapping of the trace elements was done with use of SRXRF microscopy. Iron deposits in the periportal area were more intense than those in the centrilobular area in the cirrhotic liver with NASH, suggesting that hepatocytes in the periportal area may be predominantly primed by iron-induced free radical damage.
In conclusion, elemental mapping by SRXRF microscopy was a highly sensitive method for the detection and mapping of elements such as iron in liver sections.
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| Free Research Field |
肝胆膵外科
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