2014 Fiscal Year Final Research Report
Investigation of metastasis related genes based on conditinoal knock out mouse model
| Project/Area Number |
25861190
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Digestive surgery
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| Research Institution | Hiroshima University |
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Principal Investigator |
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| Research Collaborator |
HINOI Takao (10444689)
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| Project Period (FY) |
2013-04-01 – 2015-03-31
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| Keywords | 大腸癌 / 遺伝子変異 |
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| Outline of Final Research Achievements |
We have generated an in vivo model system that Apc and Tgfbr2 were inactivated only in the colonic epithelium and tumors with well differentiated enocarcinoma arose mainly in proximal colon. Total RNAs of cancerous tissue areas were extracted , and we compared gene expression profiles of these mice’s tumors. We identified Gene X expression was most highly upregurated compared with control mice. The array data was validated by quantitative PCR. The cell proliferation assay revealed that silencing of X led to a significant reduction in CRC cell proliferation. Conversely, forced expression of X enhanced CRC cell proliferation in vitro. The analysis of this model revealed that Gene X is regulated by a TGFbeta signal and likely promotes cell proliferation in CRC.
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| Free Research Field |
大腸癌
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