2014 Fiscal Year Final Research Report
Prediction of susceptibility to anticancer agents by measuring the integrity of cell-free DNA circulating in the blood in patients with colorectal cancer
| Project/Area Number |
25861191
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Digestive surgery
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| Research Institution | Yamaguchi University |
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Principal Investigator |
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| Project Period (FY) |
2013-04-01 – 2015-03-31
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| Keywords | cell-free DNA / integrity / 大腸癌 / 化学療法 |
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| Outline of Final Research Achievements |
The circulating cell-free DNA (cfDNA) in plasma is a candidate molecular biomarker for malignant tumors. Unlike uniformly truncated DNA released from apoptotic cells, DNA released from dead cancer cells varies in size. Plasma DNA integrity, the ratio of longer fragments to shorter DNA fragment, may be clinically useful for detecting colorectal cancer progression.Patients and Methods：Plasma samples from 88 patients receiving chemotherapｙ(5-FU+Oxaliplatin) for advanced/metastatic colorectal cancer were assessed before chemotherapy. The cfDNA levels were analyzed by quantitative real-time polymerase chain reaction.Results：Cell-free DNA integrity additionally demonstrated a positive correlation to progression-free survial(184 days vs 254 days, HR of 1.93 , P = 0.03). Conclusion:cfDNA integrity measurement holds important clinical information and could become a useful tool for prediction of outcome from chemotherapy in metastatic colorectal cancer
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| Free Research Field |
消化器外科
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