2015 Fiscal Year Final Research Report
Development of a novel system for capturing circulating tumor cells (CTCs) undergoing EMT in lung cancer.
| Project/Area Number |
25861251
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Respiratory surgery
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| Research Institution | University of Occupational and Environmental Health, Japan |
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Principal Investigator |
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Keywords | 肺癌 / 循環腫瘍細胞 / 上皮間葉移行 / マイクロ流体チップ |
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| Outline of Final Research Achievements |
The “CellSerach” system is widely used to detect circulating tumor cells (CTCs), but tumor cells undergoing epithelial-mesenchymal transition (EMT) may not be captured with the “CellSearch” because an antibody against EpCAM, an antigen expressing on epithelial cells, is used to capture CTCs. Accordingly, we employed a novel microfluidic device (“CTC-chip”) in which any antibody to capture CTCs is easily conjugated, and evaluated the efficiency in capturing CTCs in experimental studies. The “CTC-chip” provided similar efficiency to “CellSeach” in capturing PC-9 cells with EpCAM expression. In capturing MESO-4 cells without EpCAM expression, the “CTC-chip” conjugated with an anti-podoplanin antibody provided a high efficiency (55-70%) whereas MESO-4 cells were not captured with the “CellSearch”.
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| Free Research Field |
胸部外科学
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