Preparation of a hybrid artificial bone combined with ex-vivo expanded bone marrow cells and an osteogenesis promoter

2015 Fiscal Year Final Research Report

• Project/Area Number: 25861306
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Orthopaedic surgery
• Research Institution: Nagoya University
• Principal Investigator: Mishima Kenichi 名古屋大学, 医学(系)研究科(研究院), 寄附講座助教 (40646519)
• Research Collaborator: KITOH Hiroshi 名古屋大学, 大学院医学系研究科, 准教授 ; MATSUSHITA Masaki 名古屋大学, 医学部附属病院, 病院助教 ; OHNO Kinji 名古屋大学, 大学院医学系研究科, 教授 ; OHKAWARA Bisei 名古屋大学, 大学院医学系研究科, 特任講師 ; OKABE Yuka (TSUKAGOSHI Yuka) 名古屋大学, 医学部附属病院, 特任助教 ; SUGIURA Hiroshi 名古屋大学, 医学部附属病院, 医員 ; HASEGAWA Sachi 名古屋大学, 大学院医学系研究科, 大学院生
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: ドラッグリポジショニング / トランスレーショナルリサーチ / 骨形成促進 / 骨折治癒促進 / 骨代謝 / 破骨細胞分化

Outline of Final Research Achievements

Soluble form of lansoprazole facilitated osteoblastogenesis more than insoluble form of lansoprazole especially at a concentration of 20 microM. Systemic administration of lansoprazole enhanced fracture healing in a rat model of femoral fracture. Implantation of lansoprazole-impregnated bone substitutes around an osteotomy site of the femurs in rats formed more calcified bones around the substitutes, whereas implantation of those into cortical bone defects of the tibiae in rabbits formed much fibrogranulation tissue surrounding the substitutes. Bone histomorphometrical analysis of the metaphyses in rats that were fed with lansoprazole for one month demonstrated significant increase of osteoclast number and function whereas that for three months did significant decrease of trabecular thickness and increase of osteoid thickness. Lansoprazole unregulated differentiation/maturation and bone absorption function in preosteoclastic cells.

Free Research Field

整形外科学