The pathogenesis of steroid induced osteonecrosis at early onset using an osteonecrosis model rat

2014 Fiscal Year Final Research Report

• Project/Area Number: 25861327
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Orthopaedic surgery
• Research Institution: The University of Tokushima
• Principal Investigator: GOTO Tomohiro 徳島大学, ヘルスバイオサイエンス研究部, 特任講師 (10420548)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Keywords: 大腿骨頭壊死 / ステロイド

Outline of Final Research Achievements

The presence of osteonecrosis was confirmed by detecting empty lacunae in histology of some specimens seven days after the last steroid administration. However, the occurrence of osteonecrosis in animal models was not constant. The positive HIF-1a was partially seen in the boundary region of the peripheral necrotic area. In MRI analysis, we have obtained enough resolution to analyze the occurrence of osteonecrosis in rat femur. However, alternative time differences between the occurrence of necrosis and detecting these lesions in MRI make difficult to do further analysis. In bio-imaging using Hypoxia probes, the positive findings in the steroid osteonecrosis rat model could not be obtained. Since it was observed variation in the creation of steroidal osteonecrosis rat model, confirmation of necrosis by image only appears to be incorrect. Thus currently, it is necessary to create more stable animal models in carrying forward this experiment.

Free Research Field

医歯薬学