2014 Fiscal Year Final Research Report
Assessment of anesthesia-induced neurodegeneration in the developing brain and clarification of this mechanism using in vivo multi-photon calcium imaging
| Project/Area Number |
25861372
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Anesthesiology
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| Research Institution | Mie University |
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Principal Investigator |
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| Project Period (FY) |
2013-04-01 – 2015-03-31
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| Keywords | 麻酔学 |
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| Outline of Final Research Achievements |
Sevoflurane in combination with propofol caused significantly greater numbers of apoptotic neurons than svoflurane alone in the neonatal mouse brain. However, there was no significant difference in apoptotic neuron density between the groups treated with sevoflurane alone and in combination with thiopental. Neurodegeneration observed in this study was not due to cardiopulmonary collapse. There is the possibility that the combination of sevoflurane and propofol is more harmful anesthetic technique than sevoflulane alone in pediatric patients. This result was published in the paper.
We were successful in multi-photon calcium imaging in the neonatal mouse cortex. However, the mechanism of neurodegeneration has not been elucidated because neonatal mice can not survive the experiment.
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| Free Research Field |
麻酔学
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