2015 Fiscal Year Final Research Report
Elucidation of central urination regulatory mechanisms through the EAAT
| Project/Area Number |
25861421
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Urology
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| Research Institution | Tottori University |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
HONDA Masashi 鳥取大学, 医学部・附属病院, 講師 (20362890)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Keywords | トランスポーター |
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| Outline of Final Research Achievements |
Intrathecal and intraventricular administration of nonselective glutamate transporters inhibitors were confirmed to suppress the micturition reflex in urethane anesthetized rats. We performed a similar experiment for each of the sub-type of glutamate transporters, but were difficult to determining the optimal dose in the drug affinity. It was reported that glutamic acid is an excitatory neurotransmitter in the micturition reflex and glycine is an inhibitory neurotransmitter. In synapse of central nervous system, the concentration of the glutamic acid and glycine are involved in urinary reflex. So, we used ALX5407, a selective inhibitor of glycine transporter GlyT-1, to access the effect of the micturition reflex in urethane anesthetized rats.
Intraventricular administration of ALX5407, there were no significant change in the micturition reflex. On the other hand, intrathecal administration, inhibition at GlyT-1 in the spinal level was elucidated to suppress the micturition reflex.
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| Free Research Field |
排尿機能
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