Investigation of the effect of the inhibitor of mitochondrial cyclophilin D activation on renal calcium crystallization.

2015 Fiscal Year Final Research Report

• Project/Area Number: 25861437
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Urology
• Research Institution: Nagoya City University
• Principal Investigator: Niimi Kazuhiro 名古屋市立大学, 医学(系)研究科(研究院), 研究員 (70551274)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: 尿路結石 / ミトコンドリア / シクロスポリン / NIM811

Outline of Final Research Achievements

We experimentally evaluated the clinical application of N-methyl-4-isoleucine cyclosporin, a novel selective inhibitor of cyclophilin D activation. In vitro, calcium oxalate monohydrate crystals induced depolarization of the mitochondrial membrane potential, which was remarkably prevented by N-methyl-4-isoleucine cyclosporin. In vivo, ethylene glycol administration induced renal calcium crystallization, oxidative stress, mitochondrial collapse and cell apoptosis in rats, which were significantly prevented by N-methyl-4-isoleucine cyclosporin.Herein we first report a new treatment agent determining renal calcium crystallization through cyclophilin D activation.

Free Research Field

尿路結石