Adenovirus-mediated Dax1 gene transfer enhances the function of Sertoli cells via the SCF/C-KIT pathway

2015 Fiscal Year Final Research Report

• Project/Area Number: 25861439
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Urology
• Research Institution: Nagoya City University
• Principal Investigator: Kurokawa Satoshi 名古屋市立大学, 医学(系)研究科(研究院), 研究員 (50468253)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: DAX1 / アデノウィルスベクター / 造精機能障害 / セルトリ細胞 / ライディッヒ細胞 / 体細胞

Outline of Final Research Achievements

(OBJECTIVE)DAX1 plays important roles in spermatogenesis in Sertoli cells in the testes. However, DAX1 function in Sertoli cells is unclear. We therefore analyzed the function of DAX1 in Sertoli cells using the adenoviral gene transfer technique.(METHODS) 1) In vitro study: An adenovirus vector was tested on the Sertoli cell line to assess the efficiency of gene transfer. The expression level of Sertoli cell-specific genes was examined by quantitative RT-PCR. 2) In vivo study: Dax1 expression in the testes was analyzed by immunohistochemistry. (RESULTS) 1) Scf was expressed at higher levels after Dax1 gene transfer. 2) Strong DAX1 staining was detected in the nuclei of Sertoli cells.(CONCLUSIONS) Strong Scf expression was observed after Dax1 gene transfer, indicating that the nuclear receptor DAX1 controls SCF expression in Sertoli cells. DAX1 may activate Sertoli cells to stimulate spermatogenesis via a SCF/C-KIT signaling pathway.

Free Research Field

泌尿器科