2015 Fiscal Year Annual Research Report
マクロファージの２つの極性に着目した結石貪食能の解析と溶解療法の開発
| Project/Area Number |
25861443
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| Research Institution | Nagoya City University |
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Principal Investigator |
田口 和己 名古屋市立大学, 医学(系)研究科(研究院), 研究員 (00595184)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Keywords | Randall's plaque / macrophage / 結晶貪食 / 結晶付着 |
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| Outline of Annual Research Achievements |
1.骨髄由来MφからM1・M2を分化誘導し、尿細管上皮細胞との共培養を行った。各Mφによる結晶の貪食率、尿細管上皮細胞への結晶付着率をそれぞれフローサイトメトリー、共焦点顕微鏡にて評価した。その結果、M1に比べM2の結晶貪食能は高く、M2との共培養により尿細管上皮細胞の結晶付着率は低下した。
2.シュウ酸カルシウム結石患者の腎乳頭部の組織を採取し、結石の原基となるプラーク(Randall Plaque＝RP)における遺伝子発現を検討した。マイクロアレイにおいて、RP部では正常粘膜部と比べて、好中球・Mφ関連遺伝子の発現増加が認められた。定量PCRにおいて、RP部位ではM1関連遺伝子であるNOS2,CSF2,IL10,CCR2,SPP1が高値で、M2関連遺伝子であるFN1,PPARG,MRC1,CD163が低値であった。
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Research Products
(11 results)
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[Presentation] The role of M1/M2 macrophages for CaOx stone and Randall’s plaque formation.2016
Author(s)
Taguchi Kazumi, Okada Atsushi, Hamamoto Shuzo, Unno Rei, Kamisawa Hideyuki, Naiki Taku, Ando Ryosuke, Umemoto Yukihiro, Itoh Yasunori, Tozawa Keiichi, Kohri Kenjiro, Yasui Takahiro
Organizer
The 31th Annual Congress of the European Association of Urology
Place of Presentation
Munich (German)
Year and Date
2016-03-11 – 2016-03-15
Int'l Joint Research
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[Presentation] Hypercalciuria and migration of inflammatory macrophages play key roles for kidney stone formation in metabolic syndrome.2015
Author(s)
Taguchi Kazumi, Unno Rei, Fujii Yasuhiro, Hamamoto Shuzo, Ando Ryosuke, Okada Atsushi, Yasui Takahiro, Fujita Keiji, Tozawa Keiichi, Hayashi Yutaro, Kohri Kenjiro
Organizer
American Urological Association Annual Meeting 2015
Place of Presentation
New Orleans (USA)
Year and Date
2015-05-15 – 2015-05-19
Int'l Joint Research
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[Presentation] Gene expression profile of Randall’s plaque in idiopathic CaOx stone formers suggests dysregulation of Mφ-related genes.2015
Author(s)
Taguchi Kazumi, Unno Rei, Fujii Yasuhiro, Naiki Taku, Hamamoto Shuzo, Ando Ryosuke, Kanemoto Kazuhiro, Okada Atsushi, Yasui Takahiro, Tozawa Keiichi, Hayashi Yutaro, Kohri Kenjiro
Organizer
American Urological Association Annual Meeting 2015
Place of Presentation
New Orleans (USA)
Year and Date
2015-05-15 – 2015-05-19
Int'l Joint Research
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[Presentation] PPAR-α/γ agonists have different effects on renal crystal formation in hyperoxaluric animal models.2015
Author(s)
Taguchi Kazumi, Unno Rei, Hirose Yasuhiko, Hamamoto Shuzo, Kobayashi Takahiro, Ando Ryosuke, Okada Atsushi, Itoh Yasunori, Yasui, Takahiro, Tozawa Keiichi, Hayashi Yutaro, Kohri Kenjiro
Organizer
American Urological Association Annual Meeting 2015
Place of Presentation
New Orleans (USA)
Year and Date
2015-05-15 – 2015-05-19
Int'l Joint Research
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