2014 Fiscal Year Final Research Report
Idntification of antigenic peptides from novel cancer stem cell antigen, DNAJB8
| Project/Area Number |
25861446
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Urology
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| Research Institution | Wakayama Medical University |
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Principal Investigator |
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| Project Period (FY) |
2013-04-01 – 2015-03-31
|
| Keywords | DNAJB8 / 癌幹細胞 / 腫瘍免疫 / ペプチド |
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| Outline of Final Research Achievements |
Three candidate peptides were identified by peptide binding assay. Of the three HLA-A24 restricted peptide, DNAJB8_22(9) (AYRKLALRW) and DNAJB8_99(9) (IFREFFGGL) induced antigen specific cytotoxic T lymphocytes (CTLs), which presented peptide specific IFN-γ secretion and cytotoxic activity. The sequence of DNAJB8_22(9) is completely identical to murine DNAJB8. Since HLA-A24 restricted peptides were tended to be compatible with binding to murine MHC class I molecule, H-2Kd, we evaluated CTL induction in vivo by immunization of DNAJB8_22(9) in Balb/c mouse model. DNAJB8_22(9) immunization could also induced DNAJB8 specific IFN-γ production and cytotoxic activity in mice.
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| Free Research Field |
泌尿器科癌
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