Development of the novel perinatal prognostic marker based on exosomal microRNA

2015 Fiscal Year Final Research Report

• Project/Area Number: 25861513
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Obstetrics and gynecology
• Research Institution: Nippon Medical School
• Principal Investigator: Mase Yuri 日本医科大学, 医学部, 助教 (20641624)
• Research Collaborator: Ishikawa Gen 日本医科大学, 医学部, 講師 (20287767)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: 産科学 / 周産期 / エクソソーム / マイクロRNA / 臍帯血

Outline of Final Research Achievements

Purpose: To utilize basic experiment of neonatal medicine, exosomal microRNA from umbilical cord blood immediately after birth was examined.
Methods: Serum blood was collected from umbilical artery and vein at the cases of uncomplicated cesarean section. Exosomes were purified from serum and exosomal RNA was extracted from exosomes. Thus, microRNAs were biotinylated, hybridized on an array and stained. After that, array signals were detected by the array scanner. Analyzing the expression intensity with analytical software. And the comparison between intensity from the umbilical artery and from vein was examined.
Results: Expression of microRNA from C19MC was observed in both umbilical artery and vein. Thus, the existence of microRNA loop traveling maternal-placental-fetal circumstances was suggested. Quantification of the expression was examined by real-time PCR on specific microRNAs which had significantly expressed with array signals.

Free Research Field

医歯薬学