2015 Fiscal Year Final Research Report
Methylation analysis by using digital PCR in head and neck cancer
| Project/Area Number |
25861546
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Otorhinolaryngology
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| Research Institution | Hamamatsu University School of Medicine |
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Principal Investigator |
Endo Shiori 浜松医科大学, 医学部附属病院, 医員 (10625205)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
|
| Keywords | Digital PCR法 / メチル化解析 / 頭頸部癌 |
|---|
| Outline of Final Research Achievements |
Bisulfite-treated DNA was amplified by PCR with two designed pairs of methylation-specific PCR primers for the promoter region of the SALL3 gene. Two
million PCR reactions using conventional TaqMan assays with a 96-well plate workflow. Three applications demonstrate that the massive partitioning afforded by our ddPCR system provides orders of magnitude more precision and sensitivity than real-time PCR. ddPCR system will allow researchers to explore complex genetic landscapes, discover and validate new disease associations, and define a new era of molecular diagnostics.
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| Free Research Field |
頭頸部癌
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