Expression of stem cell markers Oct3/4 and Nanog and its clinical significance in oral tongue squamous cell carcinoma

2016 Fiscal Year Final Research Report

• Project/Area Number: 25861587
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Otorhinolaryngology
• Research Institution: Keio University
• Principal Investigator: HABU NOBORU 慶應義塾大学, 医学部(信濃町), 共同研究員 (60365369)
• Project Period (FY): 2013-04-01 – 2017-03-31
• Keywords: 癌

Outline of Final Research Achievements

In the present study, we aimed to determine the CSC markers in the SP of HNSCC cells along with their functions in cellular behaviors, and to clarify the association of these markers with delayed neck metastasis (DNM）.Results: SP were isolated in cell lines examined. Expression levels of　Oct3/4 and Nanog were higher in SP cells than MP cells. Additionally, cell　migration and invasion abilities were higher in SP cells than MP cells, whereas there was no difference in proliferation. Univariate analysis showed that Expression of Oct3/4 and Nanog, vascular and muscular invasion, and mode of invasion were significantly correlated with DNM. Multivariate logistic regression　revealed that Oct3/4 expression and vascular invasion were independently predictive of DNM.Conclusion: These results suggest that Oct3/4 and Nanog represent probable CSC markers in HNSCC, which contribute to the development of DNM in part by enhancing cell motility and invasiveness.

Free Research Field

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