Analysis of spatial distribution of synapses and morphology of the retinal ganglion cells.

2014 Fiscal Year Final Research Report

• Project/Area Number: 25861612
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Ophthalmology
• Research Institution: Tohoku University
• Principal Investigator: MORITOH Satoru 東北大学, 医学(系)研究科(研究院), 助手 (20647234)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Keywords: 網膜神経節細胞 / 遺伝子銃 / 網膜培養

Outline of Final Research Achievements

I developed organotypic tissue culture of porcine retina with particle-mediated gene transfer. Using this method, I successfully transfected cell morphological marker plasmid and synaptic marker plasmid into the porcine retinal ganglion cells. I examined morphology and synaptic position of the porcine retinal ganglion cells. I found several types of bistratified retinal ganglion cells including a candidate for direction-selective ganglion cells in porcine retinas.

Free Research Field

分子生物学