Research for vascular anomaly growth factor by chorioallantoic membrane assay

2014 Fiscal Year Final Research Report

• Project/Area Number: 25861687
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Plastic surgery
• Research Institution: Kobe University
• Principal Investigator: EJIRI Hirotaka 神戸大学, 医学部附属病院, 助教 (70529552)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Keywords: 血管奇形 / 動静脈奇形 / 鶏卵絨毛尿膜アッセイ / 塩基性線維芽細胞増殖因子

Outline of Final Research Achievements

Most of researches for vascular anomaly have been performed by immunohistochemical study because there were be no suitable experimental animal model for vascular anomaly. Therefore we tried to use chorioallantoic membrane assay as experimental animal model for vascular anomaly.
We performed chorioallantoic membrane assay with arteriovenous malformation or basic fibroblast growth factor as experiment sample. When we applied arteriovenous malformation or basic fibroblast growth factor, there might be more angiogenesis. In our experiment, instability of chorioallantoic membrane assay was a big problem. So we have tried to change some experimental techniques, management of humidity, delivery timing of eggs, but our chorioallantoic membrane assay did not improve so much.

Free Research Field

血管腫・血管奇形