Study of double innervation in neurovascular muscle transfer

2014 Fiscal Year Final Research Report

• Project/Area Number: 25861702
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Plastic surgery
• Research Institution: Kyorin University
• Principal Investigator: SHIEN Seike 杏林大学, 医学部, 助教 (20644933)
• Co-Investigator(Kenkyū-buntansha): KURITA Masakazu Salk institute for biological studies (20424111) ; SUGA Hirotaka 杏林大学, 医学部形成外科, 助教 (60633942)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Keywords: 神経二重支配 / 筋肉移植 / 顔面神経 / 顔面神経麻痺 / 動物モデル / 神経縫合 / マイクロサージャリー

Outline of Final Research Achievements

The mechanism of double innervation of muscle has not been elucidated well. To investigate the double innervation mechanism, we have contrived a new rat model in which a segment of the latissimus dorsi muscle is reinnervated by bilateral facial nerves through proximal and distal stump of dominant thoracodorsal nerve. Electrical nerve stimulation and fluorescent retrograde tracing study were undertaken to verify that the muscle segment was reinnervated through both of bilateral mandibular branches. First, muscle contraction was evoked by electrical nerve stimulation of both sides of mandibular branches. Second, retrograde tracing studies showed fluorescent labeling of both sides of facial nuclei.
This kind of model for double innervation study has not been described to date. This model is supposed to be useful for the future manifestation of the double innervation mechanisms of transferred muscle.

Free Research Field

移植筋肉における神経二重支配