Elucidation of mechanisms of high pressure gas preservation method for clinical application in amputated limb preservation

2015 Fiscal Year Final Research Report

• Project/Area Number: 25861709
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Plastic surgery
• Research Institution: Tokyo Medical University
• Principal Investigator: Hatayama Naoyuki 東京医科大学, 医学部, 助教 (80534792)
• Research Collaborator: MATSUSHITA MASAYUKI 琉球大学, 医学部, 教授 (30273965) ; LI XIAO-KONG(KO) 国立成育医療研究センター研究所, RI管理室, 室長 (60321890) ; ARAKI JUN 東京大学, 医学部附属病院, 病院診療医 (00508088) ; NAITO MUNEKAZU 愛知医科大学, 医学部, 教授 (10384984) ; HIRAI SHUICHI 東京医科大学, 医学部, 准教授 (70516054) ; ITOH MASAHIRO 東京医科大学, 医学部, 教授 (00232471)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: 再建外科学 / 臓器保存 / 高圧ガス

Outline of Final Research Achievements

We demonstrated that exposure of rat amputated hind limbs into high-pressure medical gas without immersion in stock solution made it possible to long-term preservation. In addition, we revealed a part of mechanisms which carbon monoxide, xenon and dinitrous monoxide gas has cytoprotective effect for preservation. These results are important to show the scientific evidence and apply for clinical cases. Furthermore, we succeeded in pig amputated limb preservation by using high-pressure medical gases. This result indicates that high pressure gas preservation method can apply to organs of large animal and to have application potentiality in clinical scene.

Free Research Field

医歯薬学