Skeletal Analysys of the Twist +/- heterozygous mice, a Genetic Model for the Sathre-Chotzen syndrome

2015 Fiscal Year Final Research Report

• Project/Area Number: 25861710
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Plastic surgery
• Research Institution: Osaka Medical College
• Principal Investigator: Nuri Takashi 大阪医科大学, 医学部, 講師 (40445995)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: craniosynostosis / Saethre chotzen / coronal suture / mouse model

Outline of Final Research Achievements

Physical abnormalities such as polysyndactily were observed in all the mice which showed premature fusion after postnatal day of 42. The manner of the fusion in postnatal day of 42 were various; 20% showed part of unilateral coronal suture,40% showed partial bilateral fusion 20% showed complete bilateral fusion and 20% showed non fusion. At postnatal age of 56, 17% showed complete unilateral fusion and 83% showed complete bilateral fusion.
The 2D analysis of the distances between two landmarks wich indicate the distances of the height of the skull of Twist+/- were increased until postnatal age of 56 and the distance of the length were reached plateau at postnatal day of 28. At the postnatal age of 56, length of calvaria in WT were larger than Twist+/- and height of calvarial bone in Twist+/- were larger than WT significantly. Based on the 3D analysis, the Twist+/- shows relatively lateral displacement of the landmarks on parietal bone. The orbit were almost same between them.

Free Research Field

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