Organ protection by hypoxia response system thorough metabolic modulation

2015 Fiscal Year Final Research Report

• Project/Area Number: 25861725
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Emergency medicine
• Research Institution: Yokohama City University
• Principal Investigator: Yanagi Daisuke 横浜市立大学, 市民総合医療センター, 助教 (80638586)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: 肺傷害 / 低酸素誘導性因子

Outline of Final Research Achievements

We quantified the HIF-1α protein and ATP in the lungs of mice intraperitoneally or intratracheally treated with LPS. HIF-1α protein significantly increased at early time point (6h after the LPS challenge), and returned to the normal level within 24h in the both models. ATP concentration in lungs were also increased at early time point , however the changes were not statistically significant.
Next, we evaluated the effects of a PHD inhibitor; DMOG on LPS induced lung injury. Intratracheal DMOG treatment significantly increased HIF-1α protein in lungs, and attenuated the increase of alveolar permeability induced by intratracheal LPS administration. However, neutrophilic inflammation was not attenuated by DMOG treatment. It was suggested that the protective effects of DMOG is not mediated by suppression of neutrophilic inflammation.

Free Research Field

敗血症，肺傷害