Membrane transport protein mediatted prostaglandin E2 transport in experimentally inflmaed rat dental pulp

2015 Fiscal Year Final Research Report

• Project/Area Number: 25861794
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Conservative dentistry
• Research Institution: Niigata University
• Principal Investigator: OHKURA Naoto 新潟大学, 医歯学総合病院, 医員 (00547573)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: トランスポーター / プロスタグランジンE2 / MRP4 / PGT

Outline of Final Research Achievements

This study attempted to analyze the mRNA and protein expression of Mrp4, Pgt and EPs receptors with real-time PCR and immunofluorescent staining in lipopolysaccharide-inflamed rat incisor pulp tissue. Moreover, the amounts of PGE2 released from the inflamed pulp tissue in the presence of absence of inhibitors were assessed by using an enzyme-linked immunosorbent assay. Mrp4, Pgt, and mPGES expression were detected in the endothelial cells of normal and LPS-inflamed rat incisor pulp tissue, suggesting that these cells are associated with the biosynthesis and transmembrane efflux transport pathway of PGE2. Moreover, EP2 and EP4 were expressed in odontoblasts and endothelial cells, suggesting that EP2 and EP4 might play various roles in dental pulp inflammation by binding the PGE2.

Free Research Field

歯内療法