2014 Fiscal Year Final Research Report
The expression and function of midkine in human periapical granulomas
| Project/Area Number |
25861812
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Conservative dentistry
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| Research Institution | Nihon University |
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Principal Investigator |
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| Project Period (FY) |
2013-04-01 – 2015-03-31
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| Keywords | midkine / CXCL12 / CXCL13 / CX3CL1 / 血管内皮細胞 / 歯根肉芽腫 |
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| Outline of Final Research Achievements |
Two-color immunofluorescense image analysis indicate that co-expression of midkine(MK) and chemokine(CXCL12, CXCL13 or CX3CL1) in human periapical granulomas. Furthermore, MK and chemokines messenger RNA expression in periapical granulomas was significantly higher than healthy gingival tissues by real time PCR analysis. In addition, the MK and CX3CL1 mRNA expression in stimulated human vascular endothelial cells was significantly higher than negative control. On the other hand, CXCL12 mRNA expression was significantly lower than negative control.
These data indicated the possibility that MK, CXCL12, CXCL13 and CX3CL1 related to pathogenesis and growth of human periapical granulomas.
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| Free Research Field |
歯学
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