periodontal tissue engineering by cultured cell sheet, fibroblast growth factor 2 and beta-tricalcium phosphate collagen composite scaffold

2015 Fiscal Year Final Research Report

• Project/Area Number: 25861879
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Dental engineering/Regenerative dentistry
• Research Institution: Hokkaido University
• Principal Investigator: TAKEFU Hiroe 北海道大学, 大学病院, 助教 (40609103)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: 歯周組織再生 / β-TCPコラーゲンスキャホールド / FGF2 / 培養細胞シート

Outline of Final Research Achievements

The purpose of the present study was determine: (1)the novel method of cultured cell sheet using bone marrow cell from F344/Jcl rats’ femur; (2)the　effectiveness of the cultured bone marrow cell sheet, β-TCP collagen composite scaffold and FGF-2 on bone regeneration of rats’ calvariae.
The results were as follows: (1)the multilayered osteoblast-like cell sheet was produced by this novel method. The proper culture term made this cell sheet more rigid; (2)the complex of　β-TCP collagen composite scaffold and FGF-2 made large amount of new bone on rats’ calvariae.

Free Research Field

再生歯学