Evaluation of prospectively isolated dental pulp stem cells in vivo and application to jawbone necrosis therapy

2015 Fiscal Year Final Research Report

• Project/Area Number: 25861895
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Dental engineering/Regenerative dentistry
• Research Institution: Keio University
• Principal Investigator: YASUI TAKAZUMI 慶應義塾大学, 医学部, 共同研究員 (80348771)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: 歯髄幹細胞 / 細胞表面マーカー / フローサイトメトリー / 骨再生

Outline of Final Research Achievements

Human dental pulp stem cells (hDPSCs) isolated by plastic adherence, frequently contain contaminating hematopoietic cells and mature cells, which has limited our ability to investigate their basic biology and regenerative properties. In the present study, we prospectively isolated a pure population of hDPSCs from human dental pulp by flow cytometry. Prospectively isolated, dental pulp-derived LNGFR Low+/THY-1 High+ cells represent a highly enriched population of clonogenic cells; notably, the isolated cells exhibited high proliferation and multi-lineage differentiation potential in vitro. The cells also promoted new bone formation to heal critical-size calvarial defects in vivo. These findings suggest that LNGFR Low+/THY-1 High+ dental pulp-derived cells provide an excellent source of material for bone regenerative strategies.

Free Research Field

再生医学