Isolation and characterization of the human alveolar bones osteoblast for bone regeneration therapy

2015 Fiscal Year Final Research Report

• Project/Area Number: 25862067
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Periodontology
• Research Institution: Aichi Gakuin University
• Principal Investigator: Makoto Aino 愛知学院大学, 歯学部, 助教 (20572811)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: 歯槽骨 / 再生

Outline of Final Research Achievements

Establishment of human osteoblast cultures that retain bone forming capacity is one of the prerequisites for successful bone regeneration therapy. Because osteoblasts harvested from adults exhibit limited growth, the use of immature osteoblasts that can expand ex vivo should greatly facilitate bone regeneration therapy. In this study, we developed immature human osteoblasts isolated from aged alveolar bone (HAOBs). HAOBs obtained after the collagenase digestion of alveolar bones from elderly donors. Then, we assessed osteogenic ability of HAOB transplantation into immunodeficient mice. And, we performed global gene expression analysis to identify functional marker for HAOB. HAOBs, which can differentiate into osteoblasts and have a robust bone-forming ability, were successfully extracted from aged donors. We found that the HAOBs exhibited a higher osteogenic ability compared with those of human mesenchymal stem cells and highly expressed NEBULETTE (NEBL) with osteogenic abilities.

Free Research Field

歯周治療系歯学