Somaticmutation rate evaluation using clonal plant

2015 Fiscal Year Final Research Report

• Project/Area Number: 25870080
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Risk sciences of radiation and chemicals; Ecology/Environment
• Research Institution: Fukushima University
• Principal Investigator: Kaneko Shingo 福島大学, 共生システム理工学類, 准教授 (30635983)
• Research Collaborator: MATSUKI Yu ; INOSE Rerina ; SUYAMA Yoshihisa ; ISAGI Yuji
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: 次世代シーケンサー / 体細胞突然変異 / 低線量被ばく / モウソウチク

Outline of Final Research Achievements

The effect of radiation exposure on mutation rates is little known, especially for low doses and in situ conditions. In this study, correlations with air dose levels and DNA mutation rates were evaluated using Next Generation Sequencer for the clonal plant, Phyllostachys edulis. We collected 94 samples of Phyllostachys edulis from 14 sites with air dose rates from 0.04～7.80μSv/h. Their clonal identity was confirmed by analysis using microsatellite markers, and then, sequences among samples were compared by MIG sequence. The sequence data were obtained from 2,718 loci. In these sequences, 442 loci showed polymorphism patterns including recent origin mutation, old mutation, and sequence errors. The number of mutations per sample ranged from 0 to 13, and did not correlate with air dose levels. This result indicated that DNA mutations have not accumulated in Phyllostachys edulis living in the air doses levels less than 10μSv/h.

Free Research Field

分子生態学