Development of novel caged compounds activatable by single-photon uncaging with longer wavelength light

2014 Fiscal Year Final Research Report

• Project/Area Number: 25870180
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Physical pharmacy; Bio-related chemistry
• Research Institution: The University of Tokyo
• Principal Investigator: KAMIYA Mako 東京大学, 医学(系)研究科(研究院), 助教 (90596462)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Keywords: ケージド化合物 / BODIPY

Outline of Final Research Achievements

Caged compounds are useful tools for precise spatiotemporal modulation of cell functions, but in most cases uncaging requires ultraviolet (UV) light, which is cytotoxic and has limited tissue penetration. Therefore, caged compounds that can be activated by longer-wavelength light are required. In this research project, we describe a novel photoelimination reaction of 4-aryloxy boron dipyrromethene (BODIPY) derivatives and show that BODIPY can function as a caging group for phenol groups. We developed a novel BODIPY-caged histamine compound, which is photoactivatable with blue-green visible light to stimulate cultured HeLa cells in a spatiotemporally well-controlled manner. This caging strategy is expected to be widely applicable to develop tools for probing various cellular functions.

Free Research Field

ケミカルバイオロジー