Characterization and application of a novel dye-linked D-amino acid dehydrogenase

2015 Fiscal Year Final Research Report

• Project/Area Number: 25870271
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Applied biochemistry; Biofunction/Bioprocess
• Research Institution: University of Fukui
• Principal Investigator: Satomura Takenori 福井大学, 工学(系)研究科(研究院), 准教授 (50412317)
• Research Collaborator: SAKURABA HARUHIKO 香川大学, 農学部, 教授 (90205823) ; OHSHIMA TOSHIHISA 大阪工業大学, 工学部, 教授 (10093345) ; SUYE SHIN-ICHIRO 福井大学, 大学院工学研究科, 教授 (90206376)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: D-アミノ酸 / 色素依存性脱水素酵素 / 好熱菌

Outline of Final Research Achievements

The novel dye-lined D-amino acid dehydrogenase was found in thermophilic bacterium, Rhodothermus marinus. The primary structure of the enzyme was largely different from that of mesophilic dye-linked D-amino acid dehydrogenases so far reported. The dye-linked D-amino acid dehydrogenase was overexpressed in Escherichia coli, and its product was purified and characterized. When enzyme-catalyzed dehydrogenation of several D-amino acids was carried out using 2,6-dichloroindophenol as the electron acceptor, D-phenylalanine was the most preferable substrate among the D-amino acids tested. In addition, the enzyme was stable over a wide range of pH and did not also lose its activity by incubating 70 degrees C for 10 min. Thus, the highly thermostable dye-linked D-amino acid dehydrogenase exhibits a high potential usefulness for application to the D-amino acid bio-sensor.

Free Research Field

酵素化学