The use of mycorrhiza helper bacteria for recovering pine forest. -Resolution of the substances of mycorrhization enhancement-

2015 Fiscal Year Final Research Report

• Project/Area Number: 25870275
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Plant nutrition/Soil science; Forest science
• Research Institution: University of Yamanashi
• Principal Investigator: KATAOKA Ryota 山梨大学, 総合研究部, 助教 (00635104)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: 菌根共生促進物質 / クロマツ / 微生物間相互作用 / 根圏細菌

Outline of Final Research Achievements

The aim of this study is to purify and identify the mycorrhiza facilitation substances produced by mycorrhiza helper bacteria. Newly artificial medium was developed to purify its substance. Helper bacteria showed the enhancement of hyphal growth when valine put into the media as nitrogen source. In addition, crude substances were extracted from spent broth of helper bacteria using ethyl acetate, which showed effect of hyphal enhancement. On the other hand, newly bacteria having the hyphal enhancement activity on Fusarium oxysporum were isolated from rhizosphere soil. The sequencing analysis showed that the bacteria were Arthrobacter spp.

Free Research Field

土壌学および土壌微生物学